AssayStandard
Enzyme Activity for Line NSF3-i35-7
| Protein concn: | 54.26 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.03 | 4.59e-3 |
| C4H | cinnamic acid | 0.24 | 1.88e-3 |
| 4CL | 4-coumaric acid | 0.03 | 0.01 |
| 4CL | caffeic acid | 0.15 | 0.01 |
| HCT | 4-coumaroyl-CoA | 12.84 | 0.10 |
| C3H | 4-coumaroyl shikimic acid | 1.52 | 0.17 |
| CCoAOMT | caffeoyl-CoA | 0.17 | 0.03 |
| CCR | feruloyl-CoA | 0.18 | 0.09 |
| CAld5H | coniferaldehyde | - | - |
| CAD | coniferaldehyde | 1.39 | 0.43 |
| AldOMT | 5-hydroxyconiferaldehyde | 0.20 | 0.01 |
| CAD | sinapaldehyde | 1.64 | 0.12 |