Home

Enzyme Activity Data

The enzyme activities of the ten monolignol biosynthetic enzyme families were measured for total protein extracts of stem differentiating xylem for the wildtype and transgenic trees. Enzyme assays were performed following procedures described by Liu et al. (2012). Values show Specific Activity (nmol/min/mg). Click on the line ID for details of the assay conditions and results/statistics. If you have any questions about these data, would like to use these data for publication, or would like to collaborate, please contact Vincent Chiang (Contacts Page).

Last Updated: XXX

Family:
LineSubstrate:		PAL
Phe		C4H
CiA		4CL
4A		4CL
CA		HCT
4ACoA		C3H
4CoSA		CCoAOMT
CaCoA		CCR
FCoA		CAld5H
CnAld		AldOMT
5HCnAld		CAD
CnAld		CAD
SAld
NSF1-wt-1 0.16 0.19 0.39 1.19 16.46 11.10 5.48 3.27 0.51 0.91 4.17 23.61
NSF1-wt-2 0.11 0.24 0.38 1.52 15.20 6.37 4.18 2.55 0.41 0.98 3.50 21.33
NSF1-wt-3 0.23 0.33 0.35 1.57 15.19 12.09 5.84 1.83 0.49 1.05 3.59 19.93
NSF1-a1-1 0.14 0.30 0.29 1.06 15.12 8.59 4.84 3.16 0.51 1.05 3.05 18.20
NSF1-a1-3 0.14 0.30 0.37 1.40 16.01 8.35 5.16 2.88 0.46 1.07 4.13 23.37
NSF1-a1-8 0.16 0.27 0.26 1.40 14.39 9.64 3.21 2.71 0.48 1.07 3.61 26.65
NSF1-a2-1 0.17 0.23 0.39 1.24 16.33 7.59 5.31 2.61 0.40 1.01 3.36 24.34
NSF1-a2-3 0.14 0.22 0.29 0.62 15.28 11.73 4.93 2.20 0.36 1.06 3.87 25.61
NSF1-a2-8 0.16 0.24 0.45 1.26 17.71 14.13 5.66 4.36 0.50 1.04 3.26 24.61
NSF1-i7-2 0.04 0.30 0.52 1.14 17.82 12.60 4.79 3.80 0.47 1.04 2.43 21.70
NSF1-i7-6 0.13 0.35 0.45 0.05 16.67 11.08 4.24 3.33 0.49 1.06 2.68 22.46
NSF1-i7-8 0.07 0.39 0.46 1.07 17.59 14.19 3.20 3.24 0.49 15.26 2.65 24.89
NSF1-i7-10 0.03 0.42 0.32 1.05 19.18 14.99 6.42 3.71 0.34 14.87 2.83 15.05
NSF3-wt-1 0.02 0.16 0.08 0.34 3.70 1.48 0.19 0.26 0.42 19.92 19.91
NSF4-wt-1 0.18 0.38 0.08 2.62 4.58 3.23 1.17 1.77 5.26 16.71 21.90
NSF3-i33-2 0.04 0.31 0.12 0.35 8.53 0.65 0.27 0.14 0.69 4.60 4.00
NSF3-i33-5 0.04 0.19 0.08 0.34 5.72 1.34 0.24 0.05 0.28 3.20 4.34
NSF3-i33-10 0.04 0.17 0.05 0.22 4.64 1.54 0.31 0.23 0.29 12.73 12.83
NSF3-i34-2 0.04 0.06 0.06 0.24 5.75 4.21 0.38 0.29 0.31 25.50 22.91
NSF3-i34-8 0.04 0.08 0.09 0.10 5.73 3.39 0.18 0.27 0.43 19.23 17.04
NSF3-i34-19 0.04 0.16 0.08 0.21 6.54 3.20 0.30 0.29 0.54 20.73 18.56
NSF3-i35-1 0.05 0.14 0.09 0.42 14.95 3.33 0.24 0.23 0.31 3.31 2.83
NSF3-i35-7 0.03 0.24 0.03 0.15 12.84 1.52 0.17 0.18 0.20 1.39 1.64
NSF3-i35-15 0.02 0.09 0.06 0.23 8.96 1.03 0.21 0.23 0.28 1.83 1.46
NSF3-o65-7 0.04 0.13 0.05 0.16 3.88 2.40 0.28 0.21 0.53 7.44 12.02
NSF3-o65-11 0.04 0.19 0.09 0.17 5.28 3.17 0.18 0.19 0.47 3.92 3.97
NSF3-o65-16 0.04 0.10 0.11 0.19 5.25 2.62 0.32 0.18 0.47 3.25 3.82
NSF3-o66-7 0.04 0.06 0.05 0.15 4.10 2.33 0.14 0.16 0.45 3.11 5.12
NSF3-o66-21 0.05 0.12 0.06 0.15 5.36 2.27 0.29 0.29 0.51 3.42 6.73

 

References

Liu, J., et al. 2012. Planta. 236:879-885.