The construction of a Predictive Kinetic Metabolic-Flux (PKMF) model requires complete Michaelis-Menten and inhibition kinetic parameters for all enzymes in the pathway. We determined the Michaelis-Menten kinetic parameter (Km), the turnover number (kcat) and the catalytic efficiency (kcat/Km) for all 21 enzymes involved in monolignol biosynthesis in P. trichocarpa. Click here (Materials and Methods. If you have any questions about these data, would like to use these data for publication, or would like to collaborate, please contact Vincent Chiang Click here (Contacts Page).
Last Updated: April 12, 2015
| Family | Protein | Substrate | Product | pH | Temp oC | Km µM | Kcat min-1 | Kcat/Km µM-1min-1 |
|---|---|---|---|---|---|---|---|---|
| PAL | PAL1 | Phe | CiA | 8.5 | 37 | 32.65 ± 0.10 | 88.05 ± 4.97 | 2.70 ± 0.15 |
| PAL2 | Phe | CiA | 8.5 | 37 | 21.37 ± 0.44 | 63.93 ± 4.61 | 2.99 ± 0.16 | |
| PAL3 | Phe | CiA | 8.5 | 37 | 25.77 ± 2.71 | 93.49 ± 8.90 | 3.64 ± 0.04 | |
| PAL4 | Phe | CiA | 8.5 | 37 | 23.33 ± 1.04 | 71.68 ± 4.67 | 3.10 ± 0.32 | |
| PAL5 | Phe | CiA | 8.5 | 37 | 22.71 ± 0.13 | 75.78 ± 3.50 | 3.49 ± 0.17 | |
| C4H | C4H1 | CiA | 4A | 8.5 | 30 | 7.06 ± 0.24 | 6.72 ± 0.04 | 0.95 ± 0.01 |
| C4H2 | CiA | 4A | 8.5 | 30 | 40.70 ± 1.48 | 21.41 ± 0.46 | 0.53 ± 0.08 | |
| 4CL | 4CL3 | 4A | 4ACoA | 8 | 40 | 10.51 ± 2.84 | 44.48 ± 8.86 | 4.23 ± 1.44 |
| CA | CaCoA | 8 | 40 | 10.56 ± 2.44 | 17.07 ± 3.42 | 1.62 ± 1.44 | ||
| FA | FCoA | 8 | 40 | 70.17 ± 4.37 | 50.33 ± 2.17 | 0.72 ± 0.05 | ||
| 5HFA | 5HFCoA | 8 | 40 | 10.97 ± 1.25 | 3.08 ± 0.12 | 0.28 ± 0.03 | ||
| 4CL5 | 4A | 4ACoA | 7 | 41 | 148.06 ± 14.04 | 106.83 ± 22.75 | 0.72 ± 0.17 | |
| CA | CaCoA | 7 | 41 | 43.51 ± 5.43 | 43.69 ± 4.64 | 1.00 ± 0.16 | ||
| FA | FCoA | 7 | 41 | 133.51 ± 7.02 | 68.41 ± 17.87 | 0.51 ± 0.14 | ||
| 5HFA | 5HFCoA | 7 | 41 | 193.40 ± 21.54 | 37.34 ± 8.53 | 0.19 ± 0.05 | ||
| SA | SCoA | 7 | 41 | 508.20 ± 37.26 | 20.79 ± 5.23 | 0.04 ± 0.01 | ||
| HCT | HCT1 | 4ACoA | 4CoSA | 6.6 | 40 | 38.95 ± 4.96 | 138.35 ± 27.19 | 3.48 ± 0.28 |
| 4CoSA | 4ACoA | 6.6 | 40 | 131.13 ± 9.21 | 5.23 ± 0.19 | 0.04 ± 1.60e-3 | ||
| CaSA | CaCoA | 6.6 | 40 | 572.07 ± 31.64 | 4.29 ± 0.18 | 0.01 ± 1.00e-4 | ||
| CaCoA | CaSA | 6.6 | 40 | 23.06 ± 0.82 | 5.04 ± 0.13 | 0.22 ± 0.01 | ||
| HCT6 | 4ACoA | 4CoSA | 7.2 | 40 | 24.91 ± 0.70 | 487.86 ± 36.36 | 19.53 ± 0.93 | |
| 4CoSA | 4ACoA | 7.2 | 40 | 91.38 ± 6.19 | 29.51 ± 1.27 | 0.33 ± 0.03 | ||
| CaSA | CaCoA | 7.2 | 40 | 415.60 ± 57.74 | 19.83 ± 0.22 | 0.05 ± 0.01 | ||
| CaCoA | CaSA | 7.2 | 40 | 103.81 ± 6.44 | 58.61 ± 6.73 | 0.56 ± 0.03 | ||
| C3H | C3H3 | 4A | CA | 7 | 30 | 227.41 ± 15.62 | 67.92 ± 0.45 | 0.30 ± 0.02 |
| 4CoSA | CaSA | 7 | 30 | 4.96 ± 0.10 | 57.79 ± 0.62 | 11.65 ± 0.27 | ||
| CCoAOMT | CCoAOMT1 | CaCoA | FCoA | 7.5 | 30 | 29.78 ± 0.45 | 75.81 ± 6.14 | 2.55 ± 0.21 |
| CCoAOMT2 | CaCoA | FCoA | 7.5 | 30 | 19.10 ± 1.17 | 131.27 ± 10.38 | 6.87 ± 0.14 | |
| CCoAOMT3 | CaCoA | FCoA | 7.5 | 30 | 23.07 ± 2.58 | 44.95 ± 3.26 | 1.95 ± 0.03 | |
| CCR | CCR2 | 4ACoA | 4Ald | 6.1 | 27 | 259.81 ± 11.86 | 38.68 ± 1.90 | 0.15 ± 0.01 |
| CaCoA | CAld | 6.1 | 27 | 581.07 ± 89.67 | 78.18 ± 13.94 | 0.14 ± 0.01 | ||
| FCoA | CnAld | 6.1 | 27 | 44.89 ± 1.66 | 112.36 ± 6.29 | 2.51 ± 0.13 | ||
| CAld5H | CAld5H1 | FA | 5HFA | 5.7 | 37 | 19.30 ± 1.40 | 7.70e-4 ± 1.00e-5 | 4.00e-5 ± 3.00e-6 |
| CnAld | 5HCnAld | 5.7 | 37 | 0.05 ± 1.40e-3 | 1.23 ± 0.02 | 22.61 ± 0.69 | ||
| CnAlc | 5HCnAlc | 5.7 | 37 | 0.07 ± 1.60e-3 | 2.12 ± 0.03 | 30.90 ± 0.84 | ||
| CAld5H2 | FA | 5HFA | 5.1 | 25 | 5.20 ± 0.20 | 1.10e-3 ± 1.60e-5 | 2.11e-4 ± 9.00e-6 | |
| CnAld | 5HCnAld | 5.1 | 25 | 0.05 ± 4.00e-4 | 1.01 ± 2.00e-3 | 22.25 ± 0.20 | ||
| CnAlc | 5HCnAlc | 5.1 | 25 | 0.05 ± 4.40e-3 | 1.72 ± 0.05 | 32.67 ± 2.90 | ||
| AldOMT | AldOMT2 | CA | FA | 7.5 | 30 | 162.60 ± 8.20 | 3.00 ± 0.05 | 0.02 ± 9.80e-4 |
| 5HFA | SA | 7.5 | 30 | 9.93 ± 1.22 | 5.55 ± 0.20 | 0.56 ± 0.07 | ||
| CAld | CnAld | 7.5 | 30 | 0.14 ± 0.01 | 4.52 ± 0.14 | 32.05 ± 3.36 | ||
| 5HCnAld | SAld | 7.5 | 30 | 0.23 ± 0.07 | 2.60 ± 0.38 | 11.19 ± 3.66 | ||
| CAlc | CnAlc | 7.5 | 30 | 0.75 ± 0.04 | 6.17 ± 0.15 | 8.25 ± 0.47 | ||
| 5HCnAlc | SAlc | 7.5 | 30 | 2.46 ± 0.83 | 15.38 ± 4.17 | 6.25 ± 2.75 | ||
| CAD | CAD1 | 4Ald | 4Alc | 6.1 | 24 | 2.97 ± 0.10 | 45.89 ± 4.99 | 15.50 ± 1.69 |
| CnAld | CnAlc | 6.1 | 24 | 0.68 ± 0.04 | 30.50 ± 1.60 | 45.50 ± 2.50 | ||
| SAld | SAlc | 6.1 | 24 | 2.40 ± 0.50 | 26.90 ± 2.60 | 11.40 ± 1.00 | ||
| CAD2 | CnAld | CnAlc | 5.7 | 48 | 70.00 ± 1.30 | 110.00 ± 4.00 | 1.58 ± 0.08 | |
| SAld | SAlc | 5.7 | 48 | 35.40 ± 3.50 | 119.00 ± 11.00 | 3.37 ± 0.06 |