Specific rabbit polyclonal antibodies were synthesized for all P. trichocarpa monolignol biosynthetic enzymes. Listed below are the peptide sequences used for antibody synthesis. The peptides were designed and antibodies synthesized by Antagene Inc.
| Family | Protein | Location (AA) | Sequence |
|---|---|---|---|
| PAL | PAL2 | 630-644 | ENARLELENGNPAIP |
| PAL1 | 635-649 | SARAAYDSGNSAIEN | |
| PAL3 | 599-613 | HALENGENEKNFSTS | |
| PAL4 | 596-610 | ALVNGERERNSTTSI | |
| PAL5 | 321-335 | DGSSYVKEAQKLHEI | |
| C4H | C4H1 | 257-273 | FVDERKKLASTKNMNNE |
| C4H2 | 257-273 | FVEERKKLGSTKSMSNE | |
| 4CL | 4CL3 | 476-490 | AGEVPVAFVVKSEKS |
| 4CL5 | 476-490 | SGEIPVAFVIKSENS | |
| HCT | HCT6 | 95-109 | EAETTSVIDDFADFA |
| HCT1 | 95-109 | EAGTASVVADFGDFA | |
| C3H | C3H3 | 150-274 | MVESIFNDCTNPENNGKTLTVKKYLGAVAFNNITRLAFGK RFVNAEGVMDEQGLEFKAIVSNGLKLGASLAMAEHIPWLR WMFPLEEDAFAKHGARRDRLTRAIMDEHTLARQTSGGAKQ HFVDA |
| CCoAOMT | CCoAOMT1 | 1-15 | MATNGEEQQSQAGRH |
| CCoAOMT2 | 1-15 | MAANGEEQQTQAGRH | |
| CCoAOMT3 | 135-150 | EMLSNDKQPEFDFAFV | |
| CCR | CCR2 | 131-146 | GAVYMDPNKGPDVVID |
| CAld5H | CAld5H1 | 25-39 | FLGLISRLRRRSPYP |
| CAld5H2 | 16-30 | FLIAITSLFFLGLLS | |
| AldOMT | AldOMT2 | 1-15 | MGSTGETQMTPTQVS |
| CAD | CAD1 | 71-85 | VVGEVVEVGSDVTKF |
| CAD2 | 332-346 | YLNTAMERLAKNDVR |