AssayStandard
Enzyme Activity for Line NSF3-i35-1
| Protein concn: | 54.08 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.05 | 1.80e-3 |
| C4H | cinnamic acid | 0.14 | 2.53e-3 |
| 4CL | 4-coumaric acid | 0.09 | 0.01 |
| 4CL | caffeic acid | 0.42 | 0.02 |
| HCT | 4-coumaroyl-CoA | 14.95 | 0.12 |
| C3H | 4-coumaroyl shikimic acid | 3.33 | 0.04 |
| CCoAOMT | caffeoyl-CoA | 0.24 | 3.22e-3 |
| CCR | feruloyl-CoA | 0.23 | 0.06 |
| CAld5H | coniferaldehyde | - | - |
| CAD | coniferaldehyde | 3.31 | 0.22 |
| AldOMT | 5-hydroxyconiferaldehyde | 0.31 | 0.03 |
| CAD | sinapaldehyde | 2.83 | 0.18 |