AssayStandard
Enzyme Activity for Line NSF3-i34-2
| Protein concn: | 58.54 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.04 | 7.24e-4 |
| C4H | cinnamic acid | 0.06 | 0.04 |
| 4CL | 4-coumaric acid | 0.06 | 0.01 |
| 4CL | caffeic acid | 0.24 | 0.01 |
| HCT | 4-coumaroyl-CoA | 5.75 | 0.15 |
| C3H | 4-coumaroyl shikimic acid | 4.21 | 0.02 |
| CCoAOMT | caffeoyl-CoA | 0.38 | 0.01 |
| CCR | feruloyl-CoA | 0.29 | 0.22 |
| CAld5H | coniferaldehyde | - | - |
| CAD | coniferaldehyde | 25.50 | 0.74 |
| AldOMT | 5-hydroxyconiferaldehyde | 0.31 | 4.18e-3 |
| CAD | sinapaldehyde | 22.91 | 1.68 |