AssayStandard
Enzyme Activity for Line NSF3-wt-1
| Protein concn: | 78.7 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.02 | 3.48e-4 |
| C4H | cinnamic acid | 0.16 | 1.14e-3 |
| 4CL | 4-coumaric acid | 0.08 | 0.01 |
| 4CL | caffeic acid | 0.34 | 0.03 |
| HCT | 4-coumaroyl-CoA | 3.70 | 0.01 |
| C3H | 4-coumaroyl shikimic acid | 1.48 | 0.48 |
| CCoAOMT | caffeoyl-CoA | 0.19 | 0.01 |
| CCR | feruloyl-CoA | 0.26 | 0.02 |
| CAld5H | coniferaldehyde | - | - |
| CAD | coniferaldehyde | 19.92 | 0.46 |
| AldOMT | 5-hydroxyconiferaldehyde | 0.42 | 3.53e-3 |
| CAD | sinapaldehyde | 19.91 | 0.22 |