AssayStandard
Enzyme Activity for Line NSF1-i7-2
| Protein concn: | 8 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.04 | 0.06 |
| C4H | cinnamic acid | 0.30 | 0.04 |
| 4CL | 4-coumaric acid | 0.52 | 0.15 |
| 4CL | caffeic acid | 1.14 | 0.12 |
| HCT | 4-coumaroyl-CoA | 17.82 | 0.75 |
| C3H | 4-coumaroyl shikimic acid | 12.60 | 2.83 |
| CCoAOMT | caffeoyl-CoA | 4.79 | 0.04 |
| CCR | feruloyl-CoA | 3.80 | 0.57 |
| CAld5H | coniferaldehyde | 0.47 | 0.09 |
| CAD | coniferaldehyde | 2.43 | 0.40 |
| AldOMT | 5-hydroxyconiferaldehyde | 1.04 | 0.02 |
| CAD | sinapaldehyde | 21.70 | 4.36 |