AssayStandard
Enzyme Activity for Line NSF1-a2-3
| Protein concn: | 8 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.14 | 0.08 |
| C4H | cinnamic acid | 0.22 | 0.05 |
| 4CL | 4-coumaric acid | 0.29 | 0.07 |
| 4CL | caffeic acid | 0.62 | 0.20 |
| HCT | 4-coumaroyl-CoA | 15.28 | 0.95 |
| C3H | 4-coumaroyl shikimic acid | 11.73 | 1.06 |
| CCoAOMT | caffeoyl-CoA | 4.93 | 0.20 |
| CCR | feruloyl-CoA | 2.20 | 0.39 |
| CAld5H | coniferaldehyde | 0.36 | 0.10 |
| CAD | coniferaldehyde | 3.87 | 0.60 |
| AldOMT | 5-hydroxyconiferaldehyde | 1.06 | 0.02 |
| CAD | sinapaldehyde | 25.61 | 0.31 |