AssayStandard
Enzyme Activity for Line NSF1-wt-2
| Protein concn: | 8 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.11 | 0.05 |
| C4H | cinnamic acid | 0.24 | 0.04 |
| 4CL | 4-coumaric acid | 0.38 | 0.04 |
| 4CL | caffeic acid | 1.52 | 0.13 |
| HCT | 4-coumaroyl-CoA | 15.20 | 1.26 |
| C3H | 4-coumaroyl shikimic acid | 6.37 | 3.63 |
| CCoAOMT | caffeoyl-CoA | 4.18 | 0.59 |
| CCR | feruloyl-CoA | 2.55 | 0.36 |
| CAld5H | coniferaldehyde | 0.41 | 0.06 |
| CAD | coniferaldehyde | 3.50 | 0.66 |
| AldOMT | 5-hydroxyconiferaldehyde | 0.98 | 0.11 |
| CAD | sinapaldehyde | 21.33 | 2.65 |