AssayStandard
Enzyme Activity for Line NSF1-wt-1
| Protein concn: | 8 µg | |
| Time: | 30 min | |
| Temp: | 30 °C | |
| Buffer pH: | 7 |
Assay Results
| Family | Substrate | Specific Activity µmol/min/mg | Std. Err. |
|---|---|---|---|
| PAL | phenylalanine | 0.16 | 0.08 |
| C4H | cinnamic acid | 0.19 | 0.06 |
| 4CL | 4-coumaric acid | 0.39 | 0.02 |
| 4CL | caffeic acid | 1.19 | 0.28 |
| HCT | 4-coumaroyl-CoA | 16.46 | 1.28 |
| C3H | 4-coumaroyl shikimic acid | 11.10 | 2.99 |
| CCoAOMT | caffeoyl-CoA | 5.48 | 0.48 |
| CCR | feruloyl-CoA | 3.27 | 0.21 |
| CAld5H | coniferaldehyde | 0.51 | 0.05 |
| CAD | coniferaldehyde | 4.17 | 0.35 |
| AldOMT | 5-hydroxyconiferaldehyde | 0.91 | 0.11 |
| CAD | sinapaldehyde | 23.61 | 2.96 |