This example has the markers followed by their positions in centiMorgans. Please give all markers unique names.
-named yes -start -Chromosome 1 Marker1_1 0.0 Marker1_2 10.2 Marker1_3 34.1 Marker1_4 43.3 Marker1_5 52.1 -Chromosome 2 Marker2_1 0.0 Marker2_2 13.7 Marker2_3 19.1 Marker2_4 24.8 -stopIn the above, the line Marker1_2 10.2 means that Marker1_2 is on chromosome 1 at position 10.2 cM from the left telomere. If you want to give the chromosomes names, then the above could have been
-named yes -start -Chromosome X Marker1_1 0.0 Marker1_2 10.2 Marker1_3 34.1 Marker1_4 43.3 Marker1_5 52.1 -Chromosome First Marker2_1 0.0 Marker2_2 13.7 Marker2_3 19.1 Marker2_4 24.8 -stop
If -named had a value of no in the preamble, then the format of the distances would be:
-named no -start -Chromosome 1 0.0 10.2 34.1 43.3 52.1 -Chromosome 2 0.0 13.7 19.1 24.8 -stopThat is, the names would be skipped.
You can also create a map where the markers are not on the telomeres. If you are inputting the map based on positions, then
-start -Chromosome 1 Marker1_1 5.0 Marker1_2 10.2 Marker1_3 34.1 Marker1_4 43.3 Marker1_5 52.1 Telomere 55.3 -Chromosome 2 Marker2_1 4.0 Marker2_2 13.7 Marker2_3 19.1 Marker2_4 24.8 Telomere 27.4 -stopwould mean that there is DNA outside of the first and last markers on a Chromosome. The keyword 'Telomere' is recognized by Rmap as a telomere and not as a marker.
If you are inputting intervals, then the same map would look like
-start -Chromosome 1 Telomere 5.0 Marker1_1 5.2 Marker1_2 23.9 Marker1_3 9.2 Marker1_4 8.8 Marker1_5 3.2 -Chromosome 2 Telomere 4.0 Marker2_1 9.7 Marker2_2 5.4 Marker2_3 4.7 Marker2_4 2.6 -stop
That is, you need to indicate that the telomeric DNA comes first. These two maps should give the same result when run through Rmap.
WARNING: do not use 'Telomere' as a marker name. This keyword is case sensitive, so 'telomere' is not the same as 'Telomere'.